Introduction to cell culture splitting cells using trypsin and counting them using a hemocytometer

Course Feature
  • Cost
    Free
  • Provider
    Youtube
  • Certificate
    No Information
  • Language
    English
  • Start Date
    2020-09-02
  • Learners
    No Information
  • Duration
    No Information
  • Instructor
    Dr Germán Rosas-Acosta
Next Course
3.0
72,871 Ratings
Discover the secrets of successful cell culture in this comprehensive course! Learn how to split cells using trypsin and accurately count them using a hemocytometer. This video tutorial offers a step-by-step guide to the procedures involved in maintaining immortalized adherent human cells in tissue culture. Whether you're a beginner or an experienced researcher, this course will equip you with the essential skills needed for successful cell culture. Don't miss out on this opportunity to enhance your scientific knowledge and advance your research! Click now to enroll.
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Course Overview

❗The content presented here is sourced directly from Youtube platform. For comprehensive course details, including enrollment information, simply click on the 'Go to class' link on our website.

Updated in [September 19th, 2023]

We consider the value of this course from multiple aspects, and finally summarize it for you from three aspects: personal skills, career development, and further study:
(Kindly be aware that our content is optimized by AI tools while also undergoing moderation carefully from our editorial staff.)

What skills and knowledge will you acquire during this course?
During this course, the learner will acquire a range of skills and knowledge related to cell culture techniques, specifically the process of splitting cells using trypsin and counting them using a hemocytometer. The learner will gain a thorough understanding of the principles and importance of cell culture in scientific research and biotechnology.

The course will cover the basics of cell culture, including the different types of cells and culture vessels commonly used. The learner will become familiar with the equipment and materials required for cell culture, such as cell culture media, trypsin, and a hemocytometer.

One of the key skills that the learner will acquire is the ability to perform cell splitting using trypsin. They will learn the proper techniques for preparing and handling trypsin, as well as the steps involved in detaching cells from the culture vessel. The learner will also gain an understanding of the factors that can affect cell detachment and how to optimize the process for different cell types.

Additionally, the course will provide instruction on how to count cells using a hemocytometer. The learner will learn how to prepare a cell suspension for counting, load the hemocytometer, and accurately count the cells under a microscope. They will also learn how to calculate cell concentration and viability based on the hemocytometer counts.

Throughout the course, the learner will develop practical laboratory skills, such as aseptic technique and proper handling of cell cultures. They will also gain knowledge about the importance of maintaining sterile conditions and preventing contamination in cell culture experiments.

By the end of the course, the learner will have a comprehensive understanding of cell culture techniques, specifically the process of splitting cells using trypsin and counting them using a hemocytometer. They will be equipped with the skills and knowledge necessary to confidently perform these procedures in a laboratory setting and contribute to cell culture research and applications.

How does this course contribute to professional growth?
This course on Introduction to cell culture splitting cells using trypsin and counting them using a hemocytometer contributes significantly to professional growth. It equips individuals with essential skills and knowledge required in the field of cell culture, which is widely used in various scientific and medical research settings.

By learning the procedures involved in splitting a culture of immortalized adherent human cells, professionals can enhance their understanding of cell biology and gain hands-on experience in handling cell cultures. This practical knowledge is invaluable in many research areas, such as drug discovery, tissue engineering, and regenerative medicine.

Moreover, mastering the techniques of using trypsin and hemocytometer for cell counting allows professionals to accurately assess cell viability and proliferation rates. This skill is crucial for monitoring cell cultures, optimizing experimental conditions, and ensuring the reliability of research results.

Additionally, this course fosters a deeper understanding of the importance of maintaining sterile conditions and following proper protocols in cell culture. Professionals will learn about the potential risks associated with contamination and how to mitigate them effectively. This knowledge is essential for maintaining the integrity of cell cultures and ensuring the validity of experimental outcomes.

Furthermore, the course provides individuals with a foundation for advanced cell culture techniques and methodologies. It serves as a stepping stone for professionals to explore more complex procedures, such as transfection, differentiation, and co-culture systems. These advanced techniques are often used in cutting-edge research and can open up new opportunities for professional growth and career advancement.

Overall, this course on Introduction to cell culture splitting cells using trypsin and counting them using a hemocytometer plays a vital role in professional development. It equips individuals with practical skills, theoretical knowledge, and a solid foundation in cell culture techniques, enabling them to excel in various scientific and medical research fields.

Is this course suitable for preparing further education?
Yes, this course is suitable for preparing further education. It provides a general overview of the procedures used to split a culture of immortalized adherent human cells, which is a fundamental technique in cell culture. This knowledge will be valuable for further studies in the field of cell biology or related disciplines. Additionally, learning how to count cells using a hemocytometer is an essential skill for many research projects involving cell culture.

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