Learn DNA Primer Design for Polymerase Chain Reaction

Course Feature

Cost

Paid
Provider

Udemy
Certificate

Paid Certification
Language

English
Start Date

2021-03-30
Learners

No Information
Duration

No Information
Instructor

GHAFRAN ALI

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3.6

13,469 Ratings

This Bioinformatics course will teach you how to design DNA primers for Polymerase Chain Reaction (PCR). You will learn the steps of PCR, the specifications of primer design, and how to use Primer BLAST and Primer3 to design primers for single- or multi-insert cloning or for site-directed mutagenesis experiments. With this course, you will be able to design primers with the right GC content, length, and melting temperature to ensure accurate and efficient DNA amplification. Click now to learn the fundamentals of DNA primer design for PCR!

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Learn DNA Primer Design for Polymerase Chain Reaction

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Course Overview

❗The content presented here is sourced directly from Udemy platform. For comprehensive course details, including enrollment information, simply click on the 'Go to class' link on our website.

Updated in [August 18th, 2023]

Skills and Knowledge:
This course will provide students with the skills and knowledge to design DNA primers for polymerase chain reaction (PCR). Students will learn how to specify primer design parameters such as GC content, melting temperature, and length. They will also learn how to use Primer BLAST and Primer3 to check for specificity and design primers for single- or multi-insert cloning or for site-directed mutagenesis experiments. Additionally, students will gain an understanding of the PCR process, including denaturing, annealing, and extension steps.
Professional Growth:
This course contributes to professional growth by teaching participants how to design DNA primers for polymerase chain reaction (PCR). Participants will learn how to design primers with the correct GC content, length, and melting temperature, as well as how to avoid primer-primer annealing. This knowledge is essential for PCR experiments, and will help participants to design more efficient and accurate PCR experiments.
Further Education:
This course is suitable for preparing further education in the field of DNA Primer Design for Polymerase Chain Reaction. It covers the steps of PCR, the specification of primer design, and the importance of length, GC content, and melting temperature. It also provides a primer design tool and information on avoiding primer-primer annealing. This course provides a comprehensive overview of the topic and is a great resource for those looking to further their education in this field.

Course Syllabus

Introduction

Manual Primer Design Steps

Automated Primer Design Tool

How to validation and verified Insilco Primer design?

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